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KMID : 0848120080330040143
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International Journal of Oral Biology
2008 Volume.33 No. 4 p.143 ~ p.147
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A role of Sodium Bicarbonate Cotransporter(NBC) in HCO3 Human Salivary Gland Acinar Cells
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Jin Mee-Hyun
Park Kyung-Pyo
Hwang Sung-Min
Koo Na-Youn
Jin Mei-Hong
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Abstract
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The sodium bicarbonate cotransporter (NBC) protein is functionally expressed in salivary glands. In this experiment, we examined the role of NBC in HCO3 - formation in human parotid gland acinar cells. Intracellular pH (pHi) was measured in 2’-7’-bis(carboxyethyl)-5(6)- carboxyfluorescein (BCECF) -loaded cells. Acetazolamide (0.1 mM) and 4,4’-diisothio cyanatostilbene-2,2’-disulphonic acid (DIDS, 0.5 mM) were used as specific inhibitors of carbonic anhydrase and NBC, respectively. The degree of inhibition was assessed by measuring the pHi recovery rate (pHi/min) after cell acidification using an ammonium prepulse technique. In control experiments, pHi/min was 1.40 0.06. Treatment of cells with 0.5 mM DIDS or 0.1 mM acetazolamide significantly reduced pH/min to 1.14 0.14 and 0.74 0.15, respectively. Simultaneous
application of DIDS and acetazolamide further reduced pHi/min to 0.47 0.10. Therefore, DIDS and acetazolamide
reduced pHi/min by 19 % and 47 %, respectively, while simultaneous application of both DIDS and acetazolamide
caused a reduction in pHi/min of 67 %. These results suggest that in addition to carbonic anhydrase, NBC also partially contributes to HCO3 - formation in human parotid gland acinar cells.
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KEYWORD
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sodium bicarbonate cotransporter (NBC), human parotid gland, pHi recovery, 4, 4’-diisothio cyanatostilbene-2, 2’-disulphonic acid (DIDS), acetazolamide
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